Journal: Scientific Reports

Article Title: Long noncoding RNA lnc-RI is a new regulator of mitosis via targeting miRNA-210-3p to release PLK1 mRNA activity

doi: 10.1038/srep25385

Figure Lengend Snippet: ( a,b ) Knockdown of lnc-RI delayed M-phase progression. HeLa cells were transfected with lnc-RI RNAi-2 or Ctrl RNAi. At 12 h after transfection, the cells were treated with nocodazole (100 ng/mL) for 16 h. Mitotic cells were harvested using the mitotic shake-off method. Cell cycle profile was performed at the indicated time points after the cells were released. ( c ) The knockdown of lnc-RI induced aberrant mitotic spindle in HeLa cells. HeLa cells were transfected with lnc-RI RNAi-1, lnc-RI RNAi-2, and Ctrl RNAi and stained with anti-β-tubulin antibody and DAPI at 48 h after transfection. ( d ) The percentages of HeLa cells harbouring aberrant spindles were calculated in 200 randomly selected mitotic cells 48 h after siRNA transfection.

Article Snippet: The following antibodies were used: rabbit anti-phospho-histone H3 (S10) polyclonal antibody (A301-844A, Bethyl, USA); mouse anti-β-tubulin (D-10) monoclonal antibody (ZS-5274, ZSGB-BIO, China); mouse anti-PLK1 (F-8) monoclonal antibody (sc-17783, Santa Cruz, USA); mouse anti-β-actin monoclonal antibody (60008-1-lg, Proteintech, USA); HRP-conjugated anti-mouse IgG (074-1806, KPL, USA); FITC-conjugated anti-rabbit IgG (02-15-06, KPL,USA); and FITC-conjugated anti-mouse IgG (02-18-06, KPL,USA).

Techniques: Knockdown, Transfection, Staining

Journal: Nature Communications

Article Title: Herpesviruses mimic zygotic genome activation to promote viral replication

doi: 10.1038/s41467-025-55928-5

Figure Lengend Snippet: Reagents

Article Snippet: Β-Tubulin antibody , , Genescript , A01717-40.

Techniques:

Journal: Vaccine

Article Title: DNA vaccine encoding Middle East respiratory syndrome coronavirus S1 protein induces protective immune responses in mice

doi: 10.1016/j.vaccine.2017.02.063

Figure Lengend Snippet: Construction and verification of DNA vaccine. Schematic diagrams of the construction of DNA vaccines encoding different fragments of MERS-CoV spike protein (A). Western blot analyses of MERS-CoV spike protein expression in vitro . Lysates from pcDNA3.1-S, pcDNA3.1-SΔCD, pcDNA3.1-S1 transfected 293T cells (lane 1–3) and lysates from pcDNA3.1-Empty transfected 293T cells (lane 4) were incubated with mouse anti-MERS-S1 monoclonal antibodies and mouse anti-β-tubulin monoclonal antibodies (B). The schematic of the experiment (C).

Article Snippet: Cell lysates were prepared using RIPA Lysis buffer (Solarbio LIFE SCIENCES, Beijing, China) according to the manufacturer’s instructions, then separated on an 12% polyacrylamide gel and transferred onto a 0.45 μm nitrocellulose blotting membrane (GE Healthcare Life Sciences, Freiburg, Germany) for Western blotting analysis using mouse anti-MERS-S1 monoclonal antibodies (Sino biologicals, Beijing, China) and mouse anti-β-tubulin monoclonal antibodies (Ray antibody biotech, Beijing, China).

Techniques: Western Blot, Expressing, In Vitro, Transfection, Incubation